Ankyron CD177_HUMAN Clone CT30224

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Price £245.00
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Clone Information

Clone ?

CT30224
Gene

CD177 NB1 PRV1 UNQ595/PRO1181
UniProtID

Q8N6Q3
Organism

Homo sapiens (Human)
Entry Name

CD177_HUMAN
SNR ?

3278
Product Type

Monomer
Ankyron Applications
F, IHC, ELISA
Pack Size

100ug
Label ?
Clones are also labelled with a His tag by default
Alphalux 405 (V5, His)
Alphalux 488 (V5, His)
Alphalux 532 (V5, His)
Alphalux 555 (V5, His)
Alphalux 647 (V5, His)
Alphalux 680 (V5, His)
Biotin (V5, His)
FLAG (V5, His)
V5, His

Ankyrons® Testing Data

Immunohistochemical analysis of paraffin-embedded human liver using 10 μg/ml V5-tagged Ankyron and anti-V5 HRP (1 in 500), detecting with DAB (brown) and counterstaining nuclei with haematoxylin (blue-purple). Heat-mediated antigen retrieval with Leica Tris-EDTA buffer (pH9).
Human peripheral blood leukocytes were stained with a CD45-specific antibody directly conjugated with FITC, a CD11b-specific antibody directly conjugated with APC and 0.4 μg V5-tagged Ankyron pre-conjugated with anti-V5 APC (red). Negative control, anti-V5 APC only (grey).
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® CT30224 binding to 0.5 picomoles of immobilised CD177_HUMAN, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.

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