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Ankyron CD14_HUMAN Clone AB30169
Clone Information
|
Clone ?
|
AB30169 |
|
Gene
|
CD14 |
|
UniProtID
|
P08571 |
|
Organism
|
Homo sapiens (Human) |
|
Entry Name
|
CD14_HUMAN |
|
SNR ?
|
27639 |
|
Product Type
|
Monomer |
|
Ankyron Applications
|
F, IF, ELISA |
|
Pack Size
|
100ug |
|
Label ?
Clones are also labelled with a His tag by default
|
|
|
Ankyron Kd
|
Kd <100pM |
Ankyrons® Testing Data
Human monocytes were fixed in formaldehyde (4%), then stained using 5 µg V5-tagged Ankyron and anti-V5 antibody conjugated with APC (red) in Staining Buffer (RPMI 1640 + 10 % human serum), counterstaining nuclei with DAPI (blue). Image captured by epifluorescence microscopy.
Flow cytometric analysis of human monocytes, following incubation with 2.5 μg Ankyron and detecting with anti-V5-APC, (red). Negative control: anti-V5-APC alone, with no Ankyron present (grey).
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® AB30169 binding to 0.5 picomoles of immobilised CD14_HUMAN, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.
Affinity measurement of V5-labeled Ankyrons® AB30169 binding to CD14_HUMAN, of value Kd = 0.0976 nM, with 95% confidence bounds (CI95U, CI95L). Conjugation to ProImmune Anti-V5 HRP label reagent allows detection in Solution Equilibrium Titration immunoassay with a chemiluminescent substrate.