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Ankyron CD14_HUMAN Clone AB40381
Ankyrons® Testing Data
Flow cytometric analysis of human monocytes, following incubation with 2.5 μg Ankyron and detecting with anti-V5-APC, (red). Negative control: anti-V5-APC alone, with no Ankyron present (grey).
Titration of Ankyron clone by flow cytometric analysis of human monocytes.
Human peripheral blood leukocytes were stained with a CD45-specific antibody directly conjugated with FITC and 0.4 μg V5-tagged Ankyron pre-conjugated with anti-V5 APC (red). Negative control, anti-V5 APC only (grey).
Human peripheral blood mononuclear cells were stained with 3 μg Ankyron directly labeled with either Alphalux 405, Alphalux 488 or Alphalux 647, then gated for monocytes. Negative control, cells gated for lymphocytes (black).
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® AB40381 binding to 0.5 picomoles of immobilised CD14_HUMAN, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.