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Ankyron CCN2_MOUSE Clone CO40542
Clone Information
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Clone ?
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CO40542 |
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Gene
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Ccn2 betaIG-M2 Ctgf Fisp-12 Fisp12 Hcs24 |
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UniProtID
|
P29268 |
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Organism
|
Mus musculus (Mouse) |
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Entry Name
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CCN2_MOUSE |
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SNR ?
|
2902 |
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Product Type
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Monomer |
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Ankyron Applications
|
F, IF, ELISA, Kd |
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Pack Size
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100ug |
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Label ?
Clones are also labelled with a His tag by default
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|
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Ankyron Kd
|
Kd <10nM |
Ankyrons® Testing Data
J774A.1 mouse macrophage were fixed in formaldehyde (1%), then stained using 1.4 µg V5-tagged Ankyron and anti-V5 APC (red) in Permeabilisation Staining Buffer (RPMI 1640 + 10 % human serum + 0.5% saponin), counterstaining nuclei with DAPI (blue). Inset: negative control of anti-V5 APC alone.
Flow cytometric analysis of mouse J774A.1 cells following permeabilisation with Cytofix/Cytoperm and staining with pre-conjugated Ankyron (0.4 µg) and anti-V5 APC (red). Negative control, anti-V5 APC only (grey).
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® CO40542 binding to 0.5 picomoles of immobilised CCN2_MOUSE, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.
Affinity measurement of V5-labeled Ankyrons® CO40542 binding to CCN2_MOUSE, of value Kd = 2.73 nM, with 95% confidence bounds (CI95U, CI95L). Conjugation to ProImmune Anti-V5 HRP label reagent allows detection in Solution Equilibrium Titration immunoassay with a chemiluminescent substrate.