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Ankyron CCL5_MOUSE Clone BX40102
Ankyrons® Testing Data
J774A.1 were stimulated with TNFalpha (24 h) then fixed in formaldehyde (1%) prior to staining using 1.4 µg V5-tagged Ankyron and anti-V5 antibody conjugated with APC (red) in Permeabilisation Staining Buffer (RPMI 1640 + 10 % human serum + 0.5% saponin), counterstaining nuclei with DAPI (blue). Inset: negative control of anti-V5 APC antibody alone.
Flow cytometric analysis of TNFα-stimulated mouse J774A.1 cells, following permeabilization and staining with pre-conjugated Ankyron (0.33 µg) and anti-V5 APC (red). Negative control, anti-V5 APC only (grey).
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® BX40102 binding to 0.5 picomoles of immobilised CCL5_MOUSE, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.