Ankyron CCL4_HUMAN Clone BJ40361

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Price £245.00
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Clone Information

Clone ?

BJ40361
Gene

CCL4 LAG1 MIP1B SCYA4
UniProtID

P13236
Organism

Homo sapiens (Human)
Entry Name

CCL4_HUMAN
SNR ?

1063400
Product Type

Monomer
Ankyron Applications

,

Pack Size

100ug
Label ?
Clones are also labelled with a His tag by default
Alphalux 405 (V5, His), Alphalux 488 (V5, His), Alphalux 532 (V5, His), Alphalux 555 (V5, His), Alphalux 647 (V5, His), Alphalux 680 (V5, His), Biotin (V5, His), FLAG (V5, His), V5, His

Ankyrons® Testing Data

Monocytes were fixed in formaldehyde (1%), then stained using 1.4 µg V5-tagged Ankyron and anti-V5 APC (red) in Permeabilisation Staining Buffer (RPMI 1640 + 10 % human serum + 0.5% saponin), counterstaining nuclei with DAPI (blue). Inset: negative control of anti-V5 APC alone.
HeLa cells were fixed in formaldehyde (1%), then stained using 1.4 µg V5-tagged Ankyron and anti-V5 APC (red) in Permeabilisation Staining Buffer (RPMI 1640 + 10 % human serum + 0.5% saponin), counterstaining nuclei with DAPI (blue).
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® BJ40361 binding to 0.5 picomoles of immobilised CCL4_HUMAN, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.

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