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Ankyron CATL2_HUMAN Clone CW40421
Clone Information
|
Clone ?
|
CW40421 |
|
Gene
|
CTSV CATL2 CTSL2 CTSU UNQ268/PRO305 |
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UniProtID
|
O60911 |
|
Organism
|
Homo sapiens (Human) |
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Entry Name
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CATL2_HUMAN |
|
SNR ?
|
78500 |
|
Product Type
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Monomer |
|
Ankyron Applications
|
F, IF, ELISA |
|
Pack Size
|
100ug |
|
Label ?
Clones are also labelled with a His tag by default
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Ankyrons® Testing Data
HeLa were fixed in formaldehyde (1%), then stained using 1.4 µg V5-tagged Ankyron and anti-V5 antibody conjugated with APC (red) in Permeabilisation Staining Buffer (RPMI 1640 + 10 % human serum + 0.5 % saponin), counterstaining nuclei with DAPI (blue). Inset: negative control of anti-V5 APC antibody alone.
Flow cytometric analysis of permeabilised HeLa cells following pre-conjugation of 0.4 μg of Ankyron with anti-V5 APC (red). Negative control, anti-V5 APC only (grey)
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® CW40421 binding to 0.5 picomoles of immobilised CATL2_HUMAN, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.