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Ankyron CALR_HUMAN Clone AJ30446
Ankyrons® Testing Data
HUVEC were stained using 1.4 µg V5-tagged Ankyron and anti-V5 antibody conjugated with APC (red) in Permeabilisation Staining Buffer (RPMI 1640 + 10 % human serum + 0.5 % saponin), counterstaining nuclei with DAPI (blue) and F-actin with Phalloidin. Inset: negative control of anti-V5 APC antibody alone and F-actin staining.
HeLa cells were stained using 10 µg V5-tagged Ankyron and anti-V5 antibody conjugated with APC (red) in Permeabilization Staining Buffer (RPMI 1640 + 10 % human serum + 0.5 % saponin, counterstaining nuclei with DAPI (blue). Image captured by epifluorescence microscopy.
Colorimetric western blot analysis of purified target protein (100 - 10 ng) and cell lysates prepared from either HeLa or HCT116 cells, using 1 µg/ml V5-tagged Ankyron premixed with anti-V5-HRP antibody.
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® AJ30446 binding to 0.5 picomoles of immobilised CALR_HUMAN, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.