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Ankyron CALR_HUMAN Clone AJ30443
Clone Information
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Clone ?
|
AJ30443 |
|
Gene
|
CALR CRTC |
|
UniProtID
|
P27797 |
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Organism
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Homo sapiens (Human) |
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Entry Name
|
CALR_HUMAN |
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SNR ?
|
4977 |
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Product Type
|
Monomer |
|
Ankyron Applications
|
IF, IHC, ELISA, WB |
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Pack Size
|
100ug |
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Label ?
Clones are also labelled with a His tag by default
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Ankyrons® Testing Data
HUVEC were fixed in formaldehyde (4%), then stained using 1.4 µg V5-tagged Ankyron and anti-V5 antibody conjugated with APC (red) in Permeabilisation Staining Buffer (RPMI 1640 + 10 % human serum + 0.5 % saponin), counterstaining nuclei with DAPI (blue) and F-actin with Phalloidin. Inset: negative control of anti-V5 APC antibody alone and F-actin staining.
HeLa cells were stained using 10 µg V5-tagged Ankyron and anti-V5 antibody conjugated with APC (red) in Permeabilization Staining Buffer (RPMI 1640 + 10 % human serum + 0.5 % saponin, counterstaining nuclei with DAPI (blue). Image captured by epifluorescence microscopy.
Immunohistochemical analysis of paraffin-embedded human liver using 10 μg/ml V5-tagged Ankyron and anti-V5 HRP (1 in 500), detecting with DAB (brown) and counterstaining nuclei with haematoxylin (blue-purple). Heat-mediated antigen retrieval with Leica EDTA buffer (pH9), 40 min.
Colorimetric western blot analysis of purified target protein (1µg - 10 ng) and lysates from HeLa cells using 1 µg/ml V5-tagged Ankyron preconjugated with anti-V5-HRP (1 in 500).
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® AJ30443 binding to 0.5 picomoles of immobilised CALR_HUMAN, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.