Your basket is currently empty!
Ankyron CAH9_HUMAN Clone CU30662
Ankyrons® Testing Data
U-87 MG cells stimulated overnight with Cobalt(II) chloride hexahydrate were fixed in formaldehyde (1%), then stained using 1.4 µg V5-tagged Ankyron and anti-V5 APC (red) in Staining Buffer (RPMI 1640 + 10 % human serum), counterstaining nuclei with DAPI (blue). Inset: negative control of anti-V5 APC alone.
Flow cytometric analysis of human U-87 MG cells, either unstimulated (organge) or stimulated with the hypoxia mimic cobalt chloride overnight (red), following staining with pre-conjugated Ankyron (0.4 µg) and anti-V5 APC (orange). Negative control, anti-V5 APC only (grey).
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® CU30662 binding to 0.5 picomoles of immobilised CAH9_HUMAN, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.