Ankyron CAH9_HUMAN Clone CU30662

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Price £245.00
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Clone Information

Clone ?

CU30662

Gene

CA9 G250 MN

UniProtID

Q16790

Organism

Homo sapiens (Human)

Entry Name

CAH9_HUMAN

SNR ?

1282

Product Type

Monomer

Ankyron Applications
F, IF, ELISA
Pack Size

100ug

Label ?
Clones are also labelled with a His tag by default
Alphalux 405 (V5, His)
Alphalux 488 (V5, His)
Alphalux 532 (V5, His)
Alphalux 555 (V5, His)
Alphalux 647 (V5, His)
Alphalux 680 (V5, His)
Biotin (V5, His)
FLAG (V5, His)
V5, His
U-87 MG cells stimulated overnight with Cobalt(II) chloride hexahydrate were fixed in formaldehyde (1%), then stained using 1.4 µg V5-tagged Ankyron and anti-V5 APC (red) in Staining Buffer (RPMI 1640 + 10 % human serum), counterstaining nuclei with DAPI (blue). Inset: negative control of anti-V5 APC alone.
Flow cytometric analysis of human U-87 MG cells, either unstimulated (organge) or stimulated with the hypoxia mimic cobalt chloride overnight (red), following staining with pre-conjugated Ankyron (0.4 µg) and anti-V5 APC (orange). Negative control, anti-V5 APC only (grey).
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® CU30662 binding to 0.5 picomoles of immobilised CAH9_HUMAN, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.

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