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Ankyron C209B_MOUSE Clone AO40302
Clone Information
|
Clone ?
|
AO40302 |
|
Gene
|
Cd209b |
|
UniProtID
|
Q8CJ91 |
|
Organism
|
Mus musculus (Mouse) |
|
Entry Name
|
C209B_MOUSE |
|
SNR ?
|
761 |
|
Product Type
|
Monomer |
|
Ankyron Applications
|
IF, ELISA |
|
Pack Size
|
100ug |
|
Label ?
Clones are also labelled with a His tag by default
|
Ankyrons® Testing Data
Mouse macrophage J774A.1 cells were fixed in formaldehyde (4%), then stained using 1.4 µg V5-tagged Ankyron and anti-V5 antibody conjugated with APC (red) in either Staining Buffer (RPMI 1640 + 10 % human serum) or Permeabilisation Buffer (RPMI 1640 + 10 % human serum + 0.5 % saponin). Nuclei were counterstained with DAPI (blue) and images captured by epifluorescence microscopy. Insets: negative control of anti-V5 APC antibody alone.
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® AO40302 binding to 0.5 picomoles of immobilised C209B_MOUSE, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.