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Ankyron BATF3_MOUSE Clone CQ40509
Clone Information
|
Clone ?
|
CQ40509 |
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Gene
|
Batf3 |
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UniProtID
|
Q9D275 |
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Organism
|
Mus musculus (Mouse) |
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Entry Name
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BATF3_MOUSE |
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SNR ?
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24 |
|
Product Type
|
Monomer |
|
Ankyron Applications
|
IF, ELISA, Kd |
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Pack Size
|
100ug |
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Label ?
Clones are also labelled with a His tag by default
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|
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Ankyron Kd
|
Kd <100nM |
Ankyrons® Testing Data
J774A.1 mouse macrophage were fixed in formaldehyde (1%), then stained using 1.4 µg V5-tagged Ankyron and anti-V5 antibody conjugated with APC (red) in Permeabilisation Staining Buffer (RPMI 1640 + 10 % human serum + 0.5% saponin), counterstaining nuclei with DAPI (blue). Inset: negative control of anti-V5 APC antibody alone.
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® CQ40509 binding to 0.5 picomoles of immobilised BATF3_MOUSE, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.
Affinity measurement of V5-labeled Ankyrons® CQ40509 binding to BATF3_MOUSE, of value Kd = 11.8 nM, with 95% confidence bounds (CI95U, CI95L). Conjugation to ProImmune Anti-V5 HRP label reagent allows detection in Solution Equilibrium Titration immunoassay with a chemiluminescent substrate.