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Ankyron AURKA_HUMAN Clone CK30437
Clone Information
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Clone ?
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CK30437 |
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Gene
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AURKA AIK AIRK1 ARK1 AURA AYK1 BTAK IAK1 STK15 STK6 |
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UniProtID
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O14965 |
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Organism
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Homo sapiens (Human) |
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Entry Name
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AURKA_HUMAN |
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SNR ?
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2927 |
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Product Type
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Monomer |
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Ankyron Applications
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|
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Pack Size
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100ug |
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Label ?
Clones are also labelled with a His tag by default
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Ankyrons® Testing Data
HeLa were fixed in formaldehyde (1%), then stained using 1.4 µg V5-tagged Ankyron and anti-V5 APC (red) in Permeabilisation Staining Buffer (RPMI 1640 + 10 % human serum + 0.5% saponin), counterstaining nuclei with DAPI (blue). Inset: negative control of anti-V5 APC alone.
Immunohistochemical analysis of paraffin-embedded colorectal cancer using 10 μg/ml V5-tagged Ankyron and anti-V5 HRP (1 in 500), detecting with DAB (brown) and counterstaining nuclei with haematoxylin (blue-purple). Heat-mediated antigen retrieval with Leica Tris-EDTA buffer (pH9), 40 min.
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® CK30437 binding to 0.5 picomoles of immobilised AURKA_HUMAN, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.