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Ankyron AGRG1_HUMAN Clone AK40663
Ankyrons® Testing Data
Flow cytometric analysis of lymphocytes following pre-conjugation of 0.4 μg of Ankyron with anti-V5 APC (red). Negative control, anti-V5 APC only.
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® AK40663 binding to 0.5 picomoles of immobilised AGRG1_HUMAN, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.
Affinity measurement of V5-labeled Ankyrons® AK40663 binding to AGRG1_HUMAN, of value Kd = 28.5 nM, with 95% confidence bounds (CI95U, CI95L). Conjugation to ProImmune Anti-V5 HRP label reagent allows detection in Solution Equilibrium Titration immunoassay with a chemiluminescent substrate.