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Ankyron ACTB_HUMAN Clone CS30846
Ankyrons® Testing Data
HUVEC fixed in methanol, then stained using 1.4 µg V5-tagged Ankyron and anti-V5 APC (red) in Staining Buffer (RPMI 1640 + 10 % human serum), counterstaining nuclei with DAPI (blue). Inset: negative control of anti-V5 APC alone.
Immunohistochemical analysis of paraffin-embedded human tonsil using 10 μg/ml V5-tagged Ankyron and anti-V5 HRP (1 in 500), detecting with DAB (brown) and counterstaining nuclei with haematoxylin (blue-purple). Heat-mediated antigen retrieval with Leica Tris-EDTA buffer (pH9), 40 min.
Colorimetric western blot analysis of purified target protein (100 - 10 ng) and cell lysates prepared from either HeLa or HCT116 cells, using 1 µg/ml V5-tagged Ankyron premixed with anti-V5-HRP antibody.
Colorimetric western blot analysis of lysates from J774A.1 cells using 1 µg/ml V5-tagged Ankyron preconjugated with anti-V5-HRP (1 in 500).
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® CS30846 binding to 0.5 picomoles of immobilised ACTB_HUMAN, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.