Ankyron ACSL1_HUMAN Clone CN40119

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Price 292.00
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Clone Information

Clone ?

CN40119

Gene

ACSL1 FACL1 FACL2 LACS LACS1 LACS2

UniProtID

P33121

Organism

Homo sapiens (Human)

Entry Name

ACSL1_HUMAN

SNR ?

4656

Product Type

Monomer

Ankyron Applications
ELISA, Kd, WB
Pack Size

100ug

Label ?
Clones are also labelled with a His tag by default
Alphalux 405 (V5, His)
Alphalux 488 (V5, His)
Alphalux 532 (V5, His)
Alphalux 555 (V5, His)
Alphalux 647 (V5, His)
Alphalux 680 (V5, His)
Biotin (V5, His)
FLAG (V5, His)
V5, His
Ankyron Kd

Kd <100nM

Colorimetric western blot analysis of purified target protein (1 µg) and lysates from HepG2 cells using 1 µg/ml V5-tagged Ankyron preconjugated with anti-V5-HRP (1 in 500).
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® CN40119 binding to 0.5 picomoles of immobilised ACSL1_HUMAN, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.
Affinity measurement of V5-labeled Ankyrons® CN40119 binding to ACSL1_HUMAN, of value Kd = 40.7 nM, with 95% confidence bounds (CI95U, CI95L). Conjugation to ProImmune Anti-V5 HRP label reagent allows detection in Solution Equilibrium Titration immunoassay with a chemiluminescent substrate.

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