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Ankyron A5JS75_BOVIN Clone CL40307
Ankyrons® Testing Data
Immunohistochemical analysis of paraffin-embedded bovine spleen tissue using 10 μg/ml V5-tagged Ankyron and anti-V5 HRP (1 in 500), detecting with DAB (brown) and counterstaining nuclei with haematoxylin (blue-purple). Heat-mediated antigen retrieval at 60 °C overnight, prior to staining. Data courtesy of Dr. Benedetta Amato.
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® CL40307 binding to 0.5 picomoles of immobilised A5JS75_BOVIN, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.