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Ankyron A0A287A1C3_PIG Clone BZ30105
Clone Information
|
Clone ?
|
BZ30105 |
|
Gene
|
PDCD1 |
|
UniProtID
|
A0A287A1C3 |
|
Organism
|
Sus scrofa (Pig) |
|
Entry Name
|
A0A287A1C3_PIG |
|
SNR ?
|
1321 |
|
Product Type
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Monomer |
|
Ankyron Applications
|
F, IF, ELISA |
|
Pack Size
|
100ug |
|
Label ?
Clones are also labelled with a His tag by default
|
Ankyrons® Testing Data
L45 porcine mononuclear cells were fixed in formaldehyde (1%), then stained using 1.4 µg V5-tagged Ankyron and anti-V5 APC (red) in Staining Buffer (RPMI 1640 + 10 % human serum), counterstaining nuclei with DAPI (blue).
Flow cytometric analysis of L45 porcine mononuclear cells following fixation in formaldehyde and staining with pre-conjugated Ankyron (0.33 µg) and anti-V5 APC (red). Negative control, anti-V5 APC only (grey).
Flow cytometric analysis of L45 porcine mononuclear cells following staining with pre-conjugated Ankyron (0.33 µg) and anti-V5 APC (red). Negative control, anti-V5 APC only (grey).
Detection of porcine germinal centre T follicular helper cells in flow cytometry, using a CXCR5-specific antibody and PD-1-specific V5-tagged Ankyron preconjugated with either anti-V5 APC or anti-V5 PE. Data courtesy of Simon Graham and Daniel Zaki, Pirbright Institute.
Flow cytometric analysis of swine PBMCs following staining with V5-tagged Ankyron BZ30105 (3 µg) and anti-V5 APC-labeled Fluorotag (left panel) or Ankyron BZ30105 conjugated with FITC-Lightning Link, 0.75 µg (right panel). Data are courtesy of Prof. Falko Steinbech, University of Surrey.
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® BZ30105 binding to 0.5 picomoles of immobilised A0A287A1C3_PIG, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.