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Ankyron 4F2_HUMAN Clone DR40836
Clone Information
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Clone ?
|
DR40836 |
|
Gene
|
Slc3a2 Mdu1 |
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UniProtID
|
P08195 |
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Organism
|
Homo sapiens (Human) |
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Entry Name
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4F2_HUMAN |
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SNR ?
|
728 |
|
Product Type
|
Monomer |
|
Ankyron Applications
|
F, IF, ELISA, Kd |
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Pack Size
|
100ug |
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Label ?
Clones are also labelled with a His tag by default
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|
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Ankyron Kd
|
Kd <1nM |
Ankyrons® Testing Data
HUVEC were fixed in formaldehyde (1%), then stained using 1.4 µg V5-tagged Ankyron and anti-V5 antibody conjugated with APC (red) in Staining Buffer (RPMI 1640 + 10 % human serum), counterstaining nuclei with DAPI (blue). Inset: negative control of anti-V5 APC antibody alone.
Flow cytometric analysis of human HeLa following fixation in formaldehye and staining with pre-conjugated Ankyron (0.4 µg) and anti-V5 APC (red). Negative control, anti-V5 APC only (grey).
Flow cytometric analysis of human HeLa cells following staining with pre-conjugated Ankyron (0.4 µg) and anti-V5 APC (red). Negative control, anti-V5 APC only (grey).
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® DR40836 binding to 0.5 picomoles of immobilised 4F2_HUMAN, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.
Affinity measurement of V5-labeled Ankyrons® DR40836 binding to 4F2_HUMAN, of value Kd = 0.308 nM, with 95% confidence bounds (CI95U, CI95L). Conjugation to ProImmune Anti-V5 HRP label reagent allows detection in Solution Equilibrium Titration immunoassay with a chemiluminescent substrate.