Pro5® MHC Class I Pentamers

Prof. Eui-Cheol Shin (Korea Advanced Institute of Science & Technology, South Korea)
“ProImmune is one of the most outstanding providers of MHC Class I Multimers. Beyond their catalog of high-quality MHC Class I Pentamers, they have consistently delivered exceptional custom synthesis services tailored to my epitope requirements. Their team provided clear communication and expert guidance to design optimal MHC Class I Pentamers, which were critical to the success of my research. During the early stages of the COVID-19 pandemic, ProImmune demonstrated remarkable agility by rapidly synthesizing a broad range of SARS-CoV-2-specific MHC Class I Pentamers. Thanks to their fast turnaround time, I was able to obtain these essential reagents quickly. I ordered nearly 10 very diverse Pentamers and it worked very successfully—enabling my team to publish one of the first papers characterizing SARS-CoV-2-specific CD8+ T cells in infected patients. This work marked the beginning of my journey in SARS-CoV-2 immunology, and ProImmune played a pivotal role in that achievement.

One of the greatest advantages of working with ProImmune is their collaborative approach. Their Asia-Pacific representative keeps me updated on the latest reagents and protocols, ensuring that I’m not just purchasing a product but also receiving valuable scientific support. Before placing an order, I can consult with their specialists to discuss the best experimental protocols and reagents for my project.“

Dr. John Webb (British Columbia Cancer Agency, Canada)
“Over the past six years I have been using ProImmune’s Pro5® Pentamers for the identification and characterization of tumour epitope-specific T cells. Pentamers play an important role in my work to develop new vaccine strategies to induce T cell-mediated immune responses directed against human malignancies. I am always impressed by the knowledge and friendliness of the customer service team, and find the website very user-friendly and well-organized.”

Prof. Dirk Schlueter (Otto-von-Guericke Universitaet, Magdeburg, Germany)
“I have used ProImmune’s Pro5® MHC Pentamers in my immunology research into infectious diseases in murine models. Using the Pentamers in flow cytometry, I have found them to be of good quality and easy to use. Over the past 3 years I have had a positive experience with the company and have found them to be competitively priced and swift to manufacture and deliver their products“.

Dr. Georg Lauer (Harvard Medical School, Boston MA, USA)
“The use of peptide-coupled MHC multimers allows us to identify very rare specific cell populations with high sensitivity and specificity by flow cytometry. This was only possible for several advantages of the ProImmune Pro5® MHC Pentamer technology. Because the HCV T cell response repertoire is highly diverse, we need to have more than 20 different HCV multimers in stock in order to study a majority of our subjects. The wide range of HLA class I alleles on offer but also the opportunity to freeze the unlabelled pentamers has greatly facilitated this work. We also use many novel antibodies that are only available in a limited range of fluorochromes and here the unlabeled pentamers with the option of using different fluorotags has allowed us much more flexibility in designing our 10 color flow panels.Overall class I peptide multimers have greatly enhanced our ability to analyze human CD8 T cell responses directly ex-vivo with unprecedented specificity and sensitivity, allowing us for the first time to perform human studies that are a serious complement to studies of viral immunity in murine models.”

Dr. Daniel Volker (Institute of Immunology, Heidelberg University, Germany)
“We use ProImmune Pentamers specific for HIV epitopes to enable us to monitor the specificity and number of CD8⁺T cells in our cohort of patients over time.  The range of epitope specificities and the high quality of reagents we obtain from ProImmune are key to the success of our work.  At the moment we are really excited, as we are on the verge of publishing our findings regarding CD8⁺ T cell numbers in HIV⁺ individuals, and how these correlate with a prognosis for AIDS development.  This work has been made possible by ProImmune.”

Dr. Cath Bollard (Baylor College of Medicine, Houston TX, USA)
“Pro5® MHC Pentamers have played an important part in our study on adoptive immunotherapy for cancer and viral infections, post-transplant. We use Pentamers to detect and quantify the populations of antigen-specific T cells in CTL lines made for clinical use. We also use them to detect antigen-specific CTL in the peripheral blood of transplant patients, pre and post-CTL-infusion. The Pentamers were chosen for their stability and for the increased intensity and specificity of the positive population compared to MHC tetramers. We have found Pentamers to be consistent and reliable, and ProImmune’s customer service is excellent“.

Prof. Thomas Brocker (Institute for Immunology, University of Munich, Germany)
“ProImmune Pro5® MHC Pentamers have been influential in our research to analyze CD8 T cell responses in vivo. Colleagues in the lab recommended the Pentamers to us. For the past two years we have used them for flow cytometry applications to achieve reliable staining across different batches of the same product. I can also recommend ProImmune for their easy ordering process and excellent customer service.“