T cell epitope identification & clinical immune monitoring tools – Infectious Diseases

Wen Zhe Chua presented on T-cell epitope identification and immune monitoring tools for infectious disease research, emphasizing the importance of understanding peptide-specific T cells for protective immunity and disease progression. He described the use of Pro5® MHC Class I Pentamers and ProT2® MHC Class II Tetramers to detect, isolate, and characterize antigen-specific CD8⁺ and CD4⁺ T cells, highlighting their applications in flow cytometry, TCR sequencing, and functional assays. Case studies included early SARS-CoV-2 vaccine evaluation and HIV epitope discovery, demonstrating how these tools enable rapid, accurate identification of immunodominant T-cell epitopes.

To support a more comprehensive assessment of cellular immunity, this presentation also showcases complementary assay platforms including REVEAL® MHC-Peptide Binding Assay, ProPresent® Antigen Presentation Assays and ProSpot® ELISpot assays that together enable integrated analysis of antigen recognition, processing, and T cell activation.