Induction of SARS-CoV-2 S-specific CD8+ T cells in the lungs of vaccinated mice

Dr. Natasa Strbo from the University of Miami, discusses the use of ProImmune's Pro5 Pentamer technology in her work involving SARS-CoV-2 protein based vaccine development. Their vaccine is a cell based Heat shcok protein (Gp96-Ig) vaccine, which serves as an adjuvent as well as the antigen carrier (SARS-CoV-2 Spike protein). They used two known immunogenic epitopes from the S protein and sucessfully stained the vaccinated mouse cells with ProImmune Pro5 Pentamers, and analysed the Pentamer positive cells for intracellular cytokine secretion. With the use of Pentamers they showed an induction of S protein specicfic CD8 Tcells in lung and spleen murine cells.