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MHC Pentamers used by team at GSK to study mRNA vaccines that can protect against several Flu strains
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ProImmune launches MutaMap™ Mutational Activity Map Service
How does MutaMap™ compare to molecular evolution technologies?
Molecular evolution techniques such as phage display and other phenotype-genotype
coupled randomization techniques are most commonly used in the affinity
maturation process for monoclonal antibodies and other binding
scaffolds. The advantage of these technologies is that they help explore
a very large sequence space of combined mutations.
There comes a point
however when final decisions have to be made on the implementation of a
protein sequence where individual point mutations may be considered in
an antibody or therapeutic protein to meet a variety of design
objectives. Randomized molecular evolution is not appropriate for this
step. Exploring individual point mutations is nothing new, but it has
been difficult to carry this step out in very high throughput way,
especially where the objective is to clone and express every mutant and
then measure its affinity/activity with reasonable accuracy. This
is what MutaMap™ can achieve.
Figure 5:Example work flow for pre-clinial protein engineering of therapeutic
monoclonal antibody; individual projects may differ.
How long does a MutaMap™ project take?
Our objective is to complete medium size projects of exploring 500-2000 mutations in
approximately 8-12 weeks from receiving the customer’s protein sequence.
Larger projects will take slightly longer, depending on complexity.
What is the stepwise process for carrying out MutaMap™?
we will discuss with you the sequence space you want to explore. This
may be the known paratope of a protein or the CDR and flanking regions
of a monoclonal antibody.
To save time and cost you may not want to explore substituting amino
acids into the sequence that are considered highly non-conservative or
prone to degradation when exposed. We will also discuss what you know
about the binding interaction of your protein with its target, whether
you have working immunoassays for this interaction and whether the wild
type protein and isolated ligand is available to work with in an
immunoassay system. Depending on the nature of the interaction and the
binding partners we will ensure that the base assay of binding wild type
protein to target works well.
details are agreed and the project is initiated we will run the
mutagenesis agreed for each position, express the protein at small scale
and carry out the equilibrium binding titration affinity measurement in
What will you get?
A final technical
report delivered via our secure webserver showing you the affinity or
activity determination for the wild type and each mutant with confidence
interval. This will be presented in various formats for ease of
interpretation, including a standard heatmap.
For customers that
want to carry out protein antigenicity studies in parallel, these can be
carried out in approximately the same timeframe as the MutaMap™. This
means that within a period of approximately 8-12 weeks we will have
determined experimentally both the putative T cell epitopes and the
MutaMap™ of permitted mutations in your protein sequence. This
information can allow you to proceed with much better informed decisions
on how to address immunogenicity related issues for your program while
addressing simultaneously other developability related design decisions
for your sequence.
Introducing: ProT2™ MHC Class II Tetramers
Oxford, UK, 14 January 2016
ProImmune, a leader in services for understanding and managing adaptive
immune responses, announced today the introduction of ProT2™,
a range of Human MHC Class II tetramer reagents for tetramer analyses in
the study of antigen-specific CD4+ T cell immune responses. ProImmune’s
offering represents the broadest choice of ready to order catalog and
custom items in this field and will enable research into CD4+ T cell
responses in a variety of disease areas including cancer, infectious
diseases, allergy, autoimmunity and transplantation. Another key area
for this product range is the detailed study of T cell help in unwanted
immune responses to biologics.
CD4+ T cell
responses are at the heart of steering the adaptive immune system
directing both cytotoxic T cell responses and antibody responses
mediated by B cells. ProT2™ MHC Class II Tetramers allow the direct
detection and separation of single antigen-specific CD4+T cells
accurately by flow cytometry.
Schwabe, CEO of ProImmune commented: “By introducing a broad and growing
ProT2™ Tetramer product range we intend to give MHC Class II tetramer
analysis the emphasis it deserves in the study of CD4+ T cell responses
which play such a pivotal role in adaptive immunity across so many
disease and treatment modalities.”
Class II MHC Monomer reagents are supported by ProImmune’s expert
customer service and application support, helping new users in
particular to establish and optimize robust protocols in their own
addition to ProImmune’s class II reagent offering further complements an
already extensive range of services for evaluating CD4+ T cell
responses, including ProImmune REVEAL® MHC peptide binding assays, CD4+
T cell and DC T cell proliferation assays and standard immune monitoring
services through ELISPOT and flow cytometry based assays.
information on ProT2™ reagents and their availability, or to place an
order, please visit:
, or call + 1 888 505 7765 in the United States or + 44 870 042 7279 in other countries.
Notes to Editors:
About ProImmune -
ProImmune’s mission is to
be a partner of choice for understanding and managing adaptive immune
responses. It does this by offering unique solutions for preclinical and
clinical immunology research, including a comprehensive and integrated
antigen characterization and immunogenicity testing platform, and
products and services for tracking antigen-specific immune responses
with state-of-the-art ELISpot and flow cytometry techniques. ProImmune
is committed to helping researcher’s achieve success through product
innovation, responsive service and focused application support, saving
time and money, and reducing risk.
For more information, please contact
Tel: +44 (0) 870 042 7279
Zyme Communications Ltd
Tel: +44 (0) 7771 730 919
ProImmune Introduces ProSentium™ a Unique Peptide Sequence Database to Investigate T Cell Immune System
Oxford,UK, 14 April 2015
– ProImmune, a leader in services for understanding and managing
adaptive immune responses, announced today the introduction of a new
antigen database service called ProSentium™, based on
assay results using sequencing mass spectrometry. ProSentium data
can show the precise peptide sequences from proteins of interest
visible to the body’s own T cell immune system. The data
collection covers many high value therapeutic targets and areas including the
majority of licensed replacement factor proteins, including clotting
factors, replacement enzymes, spanning development areas including
oncology, cardiovascular, CNS, immunology, gastroenterology and many
Under an exclusive or non-exclusive license, the bespoke database service
investigates and reports peptide hits against the ProSentium
database for a customer’s specific protein or protein family of
interest. Results are reported as overlapping nested peptide sets
and provide statistically relevant antigen sequence data that can be
used to further inform drug development decisions.
CEO of ProImmune commented: “ProSentium supports pivotal decision
making in drug design and development with the physical evidence
required for game changing approaches that target or interact with
the immune system. Through our service offering, we are delighted
that we can offer this ground-breaking resource to researchers so
they can better understand the body’s immune response and bring new
therapies to patients. ”
T cells play a
critical role in all immune responses, including in fighting
infections, cancer and in autoimmunity and in unwanted drug
reactions. To understand T cell responses, it is crucial to
understand the specific peptide antigens that the immune system
recognizes and are presented on the cell surface of both normal and
diseased cells. ProSentium data can be readily analyzed and
reported, and is fully compatible with ProImmune’s in vitro
assay services which can be combined to help investigators gain a
more complete understanding of relevant immune responses at a
For more information on ProSentium please visit:
, or call +1 888 505 7765 in the United States or +44 870 042 7279 inother countries.
More on ProSentium™
About ProImmune -
ProImmune’s mission is
to be a partner of choice for understanding and managing immune
responses. It does this by offering unique solutions for preclinical
and clinical immunology research, including a comprehensive and
integrated antigen characterization and immunogenicity testing
platform, and products and services for tracking specific immune
responses. ProImmune is committed to helping researcher’s achieve
success through product innovation, responsive service and focused
application support, saving time and money, and reducing risk.
18 Nov 2019 To 22 Nov 2019
PEGS Europe 2019, Lisbon
21 Oct 2019 To 25 Oct 2019
Immunogenicity & Bioassay Summit, VA
09 Sep 2019 To 10 Sep 2019
Mastering Immunity 2019, Museum of Science, Boston
05 Aug 2019 To 09 Aug 2019
Immuno-Oncology Summit, Boston
“Format and Interpretation of a Drug Tolerant Approach for Measuring Circulating Immune Complex and Free Anti-Drug Antibodies in Preclinical Studies”
“In silico prediction of immunogenicity: filling the gaps”
“An Integrated Approach to Managing Immunogenicity Risk and Optimum Protein Design”
“Use of the MHC Associated Peptide Proteomic (MAPPs) assay to understand clinical outcomes associated with the use of Factor VIII in the treatment of Hemophilia A”
“Measuring Host-vs-CAR-T Immune Responses”
“Epitope identification and clinical immune monitoring in gene therapy and immune oncology program”
“De novo generation of tumor specific T cell responses utilizing a novel oncology driven delivery platform”
“Direct visualisation of antigen-specific CD4+ T cells: insights from HLA class II tetramers”
“Comprehensive analysis of pre-existing T cells specific to therapeutic proteins in healthy donors”
“Precision Medicine in Transplantation – immune monitoring for the discrimination of GVHD, rejection and CMV infection”
“Phosphopeptide NeoAntigens for Tumor Immunity: Novel therapeutic options”
“Heterologous prime-boost approaches to CD8+ T cell induction”
“Host microbe interactions in the intestine: New therapeutic strategies in IBD”
“Personalized Cancer Vaccines”
“Next Generation approaches to enhance the efficacy of SPEAR T-cells”
“Potent cellular and humoral immunogenicity of a pre-erythrocytic viral vectored malaria vaccine in African infants and children”
“So You Think You Have Mastered Immunity? Cautionary Tales from the Immunology Revolution”
“Use of oncolytic parvoviruses in the treatment of malignant gliomas: a viro-immunotherapeutic approach”
“Tregs -ready for the clinic”
Dr Jeremy Fry provides a summary of ProImmune’s REVEAL and ProVE® service, the rapid epitope discovery system.
Dr Andrew Waller provides an overview of the Pro5 Pentamer technology and the benefits of using MHC pentamers to detect specific CD8 T Cell populations in your research.
Dr Heather Hensler introduces ProArray Ultra®, the high-throughput peptide and protein microarray assay service.
Dr Briana Betz explains the importance of understanding the HLA types of your study cohort, and how ProImmune can help you.
Dr Nikolai Schwabe introduces the ProPresent antigen presentation assay and some of its applications in the field of immunogenicity.
Managing immunogenicity risk should be a core focus in drug development. ProImmune’s CEO Nik Schwabe explains why.
thinkpeptides Peptide Ebook
Chulalongkorn University, Thailand
"I outsourced my tissue typing to ProImmune because they provide
me with the fastest service at the best price. Since the main
theme of my research is on T cell immunology, HLA-type is
inevitably needed. The customer service from ProImmune is of the
best quality. Their typing service allowed me to unambiguously
resolve the HLA type of many of my samples."
Duke Human Vaccine Institute, North Carolina, USA
"We have a programme of clinical studies and it is important for
our researchers to be able to correlate the immune responses
they observe with HLA type. We looked at a range of companies
offering tissue typing, and also at using an on-campus typing
facility, and we found that ProImmune were able to offer us the
best pricing and turnaround time. We now send them regular
batches of samples to type. They have been wonderfully easy to
work with, if we ask anything they get right back to us and
overall it has been a really good experience."
New York Medical College, New York, USA
Prof. Dattwyler used ProImmune's B cell Linear Epitope Mapping
Service to discover the linear epitopes present in two different
proteins. "I was really happy with the way that these guys took
the trouble to adjust my quote so that I had the best assay
design for the lowest price. They were really easy to work with,
always replied to my questions, and delivered the report on my
project on schedule as promised. It's great that I've been able
to go back to them and order my new epitopes as custom peptides
for follow on work, and I'm going to revisit my validated
epitopes with ProImmune's help and get some really fine mapping
of antibody binding to these sequences."
HLA Tissue Typing
ProImmune REVEAL & ProVE® Epitope Discovery
ProMap® T Cell Proliferation Assay