Tier 1 Typing using PCR-SSOP, resolves major allele groups to 4 digits,
with some degeneracy
Tier 2 Typing using PCR-SSP or PCR-sequencing for higher resolution
Order any or all of the following loci: Class I (A, B, C) and Class II
(DRB1, DRB3/4/5, DPB1, DQB1)
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Detailed results typically
sent in 20
working days
Why should you use it?
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Professional, reliable HLA Tissue Typing service
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Avoids issues that can arise from informal relationships with a local typing lab
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In advance of starting functional cellular
assays such as ELISPOT, or carrying out flow cytometry testing with HLA
multimers, tissue typing is usually required for interpreting results
successfully
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If you are carrying out a clinical trial,
establishing the tissue type of your donor samples is an important step.
Many diseases and the immunological response to them are associated with
specific HLA types. Correlating your trial results with the sample tissue
type may give you critical additional information that could make a
difference to the interpretation of the results
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If your time and resources are limited, and deadlines are
imminent, our typeHLA Service
provides just the help you need
The typeHLA service in detail
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Tier-1, Class I and Class II
Typing by PCR-sequence specific
oligonucleotides (PCR-SSOP) to resolve major allele groups to 4 digits,
with some degeneracy e.g. HLA-A*23:01 / 03/ 05/ 06). PCR-SSOP: The genomic
DNA is amplified using PCR, then incubated with a panel of different
oligonucleotide probes, which have distinctive reactivities with different
HLA-types. The Luminex xMAP® technology is used, where oligonucleotide
probes are individually attached to up to 100 distinctly fluorescent
microspheres. This allows the measurement of 100 different reactions in a
single tube.
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Tier 2, Class I and Class II
Can be carried out if needed following Tier
1 typing to achieve higher resolution. Typing by PCR-sequence specific
primers (PCR-SSP) or PCR-sequencing, will usually resolve to a specific
4-digit allele, with only occasional degeneracy. PCR-SSP: the PCR reaction
is used to define whether the targeted HLA allele is present or
absent by using reagents in the PCR reaction specific for individual HLA
alleles. PCR-sequencing: the DNA
sequence of the HLA allele can be directly analyzed by performing
nucleotide sequence analysis of the amplified DNA.
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Sample types and order handling
We accept genomic DNA, fresh whole
blood or frozen cells, and will provide you with details of how to ship
each of these to us. There is an order handling charge for samples sent as
whole blood or frozen cells due to the additional processing needed for
these sample types.
We offer a worldwide service, available for one sample or hundreds - our
high throughput service enables us to process tens to hundreds of samples
at a time. You can confidently send your samples from any location
worldwide using our experience in shipping globally. We can accept genomic DNA,
whole blood or cryopreserved PBMCs.
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Discuss your requirements with our customer service team of
highly trained immunologists. They will work out the best service
for you, and manage the whole process from sample handling to
delivery of the final report. Typically, the results report is available 20 working days following
receipt of the samples and is sent by email to the client. ProImmune's Cellular Analysis Services
ELISPOT
assays, Intracellular Cytokine Staining, CFSE T Cell Proliferation assays
and Flow Cytometry staining with MHC Class I Pentamers. |
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