Protocols | ProImmune

Protocols

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Pro5® MHC Class I Pentamer Staining Protocols

Labeled Pentamer staining protocol

Unlabeled Pentamer staining protocol
(applicable for standard unlabeled Pentamers and ProVE® Pentamers)

Biotin-labeled Pentamer staining protocol

Pentamer staining for whole blood

Staining a single cell sample with multiple labeled Pentamers

Staining a single cell sample with multiple unlabeled Pentamers

Pentamer immunohistochemistry protocol

 

Co-staining of Pentamers and Intracellular Cytokines

Human Pentamer and intracellular cytokines

Murine Pentamer and intracellular cytokines

 

CD1d Tetramer Staining Protocols

CD1d Tetramer staining protocol

 

Protocols for Cell Separation using Magnetic Beads

Guide to Cell Separation using Magnetic Beads

Column-based bead isolation for fluorescent Pentamers

Column-based bead isolation for biotin-labeled Pentamers

Tube-based bead isolation for biotin-labeled Pentamers

CD137 isolation and staining protocol

 

Cell Preparation Protocols

Protocol for red blood cell depletion from whole blood

Protocol for density gradient separation of human PBMCs from blood

Protocol for the isolation of splenocytes from murine spleens

Cryopreservation of cell samples

Protocol for the isolation and expansion of antigen-specific cells

 

Protocols for Peptide Use

Storage and solubilization of peptides

PEPscreen®: Custom Peptide Libraries Storage and Handling Guidelines

Guidelines for dissolving PEPscreen® Peptide Sets