ProImmune Pre-Mixed Peptide Pools

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ProMix™ Peptide Pools

purified immunodominant epitope peptide pools for precise monitoring of functional T cell responses

Image: HIV gag p17&p24 protein complex

Figure 1. HIV-1 gag p17/p24 with three epitopes from the HIV ProMix® peptide pool mapped to its surface.

Choose  ProMix™, the smarter peptide pool for smart immunology

ProMix™ peptide pools from thinkpeptides consist of a select number of peptides representing the key immunodominant epitopes from a specific protein or pathogen, providing a comprehensive picture of the T cell response. Peptide selection is based on well-established and published epitopes with a wide range of HLA restrictions covering the most relevant HLA types in the population.

Unlike peptide pools of protein spanning overlapping peptide libraries ProMix pools allow for a much greater control and purity of its constituent peptide components and avoid overloading cell cultures with large numbers of irrelevant non-responding peptides that can contribute excessive contaminants from peptide synthesis processes.

 

Key advantages of ProMixes™:

  • ProMix™ peptide pools use well characterized epitopes to provide a representative picture of the T cell response against a protein or disease.

  • Smaller numbers of peptides in each pool are designed to elicit a targeted response based on immunodominant epitopes.

  • Uniform >95% purity in pools.

  • Peptide pool content is clearly defined, allowing individual peptide responses to be mapped easily if this is required.

  • Highly efficient and cost effective method for classifying T cell response.

 

Example Published Data generated with ProMix Peptide Pools

Anikeeva N et al. (2016). "Evaluating frequency and quality of pathogen-specific T cells"

Nature Communications DOI: 10.1038/ncomms13264

Brief synopsis: Using synthetic peptides and peptide pools Anikeeva et al. have developed a Ca2+ signalling activation assay that offers new insghts into the dynamics of antigen-specific T cell activation.

 Figure 1. Kinetics of calcium response of CMV-specific CD8+ T cells. CMV-specific CD8+ T cells from a healthy donor (a) and a patient after haploidentical stem cell transplantation (b) revealed different kinetics of Ca2+ signalling on antigen stimulation. CD8+ T cells were purified from PBMC by negative magnetic sorting and purified CD8 T cells were labelled with Fluo-4 and immobilized on a glass surface as described in ‘Methods' section. The T cells from healthy donor were stimulated by CMV-derived peptide NLVPMATV at 10−4 M while the T cells from the patient were triggered with ProMix CMV peptide pool at 9 × 10−5 M. The images of responding cells are taken at 720 s after the stimulation. The data shown are representative of 5 (a) and 2 (b) independent experiments and are based on the analysis of 4,600 and 4,200 T cells, correspondingly. Scale bars, 50 μm.

 

Research Areas

Cancer

ProMix™ Number of peptides Code
ProMix™ NY-ESO-1 Peptide Pool 5 PX-NYESO
ProMix™ Prostate Specific Antigen Peptide Pool 9 PX-PSA
ProMix™ Survivin Peptide Pool 11 PX-SURV
ProMix™ bcr/c-abl Peptide Pool 6 PX-BCR
ProMix™ Telomerase Reverse Transcriptase Peptide Pool 12 PX-TERT
ProMix™ Prostate Specific Membrane Antigen Peptide Pool 7 PX-PSMA
ProMix™ gp100/p-mel Peptide Pool 8 PX-GP100
ProMix™ Tyrosinase Peptide Pool 6 PX-TYR

 

Infectious Diseases

ProMix™ Number of peptides Code
ProMix™ Adenovirus Peptide Pool 5 PX-ADENO
ProMix™ CMV Peptide Pool 14 PX-CMV
ProMix™ EBV Peptide Pool 26 PX-EBV
ProMix™ Influenza Peptide Pool 17 PX-FLU
ProMix™ Hepatitis C Virus Peptide Pool 21 PX-HCV
ProMix™ Hepatitis B Virus Peptide Pool 9 PX-HBV
ProMix™ HIV Peptide Pool 22 PX-HIV
ProMix™ Human Papillomavirus Peptide Pool 14 PX-HPV
ProMix™ CEFT Peptide Pool 24 PX-CEFT
ProMix™ CEF Peptide Pool 32 PX-CEF
ProMix™ Respiratory Syncytial Virus Peptide Pool 28 PX-RSV

 

 

In most cases we aim to achieve coverage for the most common HLA types, and where applicable other alleles that may be of particular relevance with the disease or protein of interest.

With ProMix™ peptide pools, the pooling procedure is done for you, with the peptides guaranteed >95% pure. Each ProMix™ pool contains 25μg of each peptide and is available for dispatch within 1-2 days.

HLA-A HLA-B
A*01:01 B*07:02
A*02:01 B*08:01
A*03:01 B*35:01
A*24:02 B*27:05
A*11:01 B*44:03
A*68:01 B*57:01
B*51:01

 

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