Prospector™ Custom Peptide Libraries | ProImmune

Prospector™ Custom Peptide Libraries

Synthetic custom peptides offer a rational, scaleable and ever more affordable approach for exploring protein-protein interactions or even more complex phenomena such as immune responses directed against specific epitopes. As the use of such peptides increases it becomes ever more important to consider all relevant factors when designing large peptide libraries for synthesis. Our applications scientists have the experience to help you get the peptide design right for your project. Our approach is to offer you flexible, scaleable synthesis wherever you are, with a wide range of peptide library formats to choose from.

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Prospector™ Pure Purified Peptide Libraries for Exacting Applications

Our Prospector™ Pure purified peptide libraries deliver on the most exacting requirements and at prices that help you break new ground on research driven by synthetic peptides. Unlike the synthesis of individual purified peptides, these libraries are made using a parallel synthesis platform that guarantees less product variance and delivery in an easy to handle tube-array format. Our high throughput purification combined with 100% LC-MS analysis ensures that you do not have to lose critical path time on your projects just because you need to work with purified and fully analyzed peptides. Our technology also has the flexibility to meet multiple design parameters, such as different peptide lengths, N- and C-terminal modifications and non-standard amino acids.

Prospector™ Peptide Libraries with High Average Purity

Prospector™ Peptide Libraries deliver highly attractive prices and exacting 100% LC-MS quality control. Though these libraries are not purified, they are synthesized using the same reliable synthesis platform as our purified products and the resulting peptides have high average purity. For example, for Prospector PEPscreen®, the average purity of 10mers is ~86%, the average purity of 15mers is ~73% and the average purity of 20mers is ~61%. The features of Prospector™ Libraries make them an ideal product for epitope discovery applications, giving you the comfort of comprehensive QC so you know absolutely what you are working with.

Unlike other commercial peptide libraries available, there are no hidden set-up charges. A single price is charged per peptide, and the order size starts at only 24 peptides. Prospector PEPscreen®: Custom Peptide Libraries accommodate peptides from 6-20 amino acids; information in the table below about all other Prospector™ libraries is based on peptides from 7 to 15 amino acids; peptides up to 40 amino acids can be made using the Prospector™ technology platform, but quantities and dispatch times will vary, depending on the specification.

Prospector™ libraries are perfect for researchers using large numbers of peptides in applications such as CD4+ and CD8+ T cell epitope mapping, B cell epitope mapping, ELISPOT, intracellular cytokine staining, protein-protein interactions, receptor-ligand studies and cellular assays.

Comprehensive Range of Prospector™ Peptide Libraries

Code Description Purity Quantity QC Estimated
Dispatch
PP97 Prospector™ Pure97 >97% >1 mg 100% LC-MS 3-4 weeks <200
PP95 Prospector™ Pure95 >95% >1 mg 100% LC-MS 3-4 weeks <200
PP90 Prospector™ Pure90 >90% >1 mg 100% LC-MS 3-4 weeks <200
PP80 Prospector™ Pure80 >80% >1 mg 100% LC-MS 3-4 weeks <500
PP70 Prospector™ Pure70 >70% 1-5 mg 100% LC-MS 3-4 weeks <500
PPLC Prospector™ LCMS Crude 1-5 mg 100% LC-MS 2-3 weeks <500
P0L Prospector PEPscreen® Crude 0.5-2 mg 100% MS or
100% LC-MS
2-3 weeks <500
PPLX Prospector™ Lightning-X
Peptides with C-terminal amino acid of choice
(Min. order 384 peptides)
Crude 50-100 nmol 5% LC-MS 2-3 weeks <10,000
PPLG Prospector™ Lightning-G
Peptides with C-terminal Glycine
(Min. order 384 peptides)
Crude 50-100 nmol 5% LC-MS 2-3 weeks <10,000

Quality Control

The power of high throughput LC-MS analysis
LC-MS means analysis by combined sequential liquid chromatography and mass spectrometry. Liquid chromatography is used to determine different species in each peptide and what proportion each species has overall. Mass spectrometry determines the molecular mass of a species and indicates by implication the molecular composition of a species. LC-MS quality control determines the mass spec of the major peak found by liquid chromatography. Combining the two methods means that it is possible to determine with certainty that the purified peptide is the species of interest. Peptides that fail QC will be remade once. If a peptide still does not reach it's specification the customer will be contacted to discuss options for re-synthesis.

For the Prospector PEPscreen® product, MALDI-TOF Mass Spectrometry (MS) is performed on 100% of samples.  Each peptide must meet both the MS analysis and the final gross weight criteria to pass quality control (QC).  For a peptide to pass the MS criterion the desired molecular mass must be one of the three major ions.  Peptides that fail QC by MS will be remade once.  Subsequent failed peptides are supplied as part of the order, and are labeled accordingly in the paperwork, allowing the user to decide whether or not to include the peptide in their studies.

Product Format

Prospector™ Pure, Prospector™ LCMS and Prospector PEPscreen® Custom Peptide Libraries

The peptides are dried as a thin film at the bottom of individual tubes. This is to prevent the peptide from smearing throughout the inside of the tube during transit, which can make resuspension in small volumes difficult. Tubes are individually capped and arranged in a standard 8 x 12 tube array for compatibility with high throughput assays. This format also allows the flexibility to select only the tubes of peptides of interest and rearrange them into a convenient assay format. Each tube is clearly labeled in case the tubes are accidentally mixed.
Minimum order size 24 peptides.

Prospector™ Lightning Custom Peptide Libraries

The peptides are dried as a thin film in a 96-well plate, with clear labeling for peptide set identification.
Minimum order size 384 peptides.

 

thinkpeptides is a brand of ProImmune
PEPscreen is manufactured by Sigma (formerly Sigma-Genosys) for ProImmune
PEPscreen is a registered trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.